Publication date: 1 May 2026
Source: Nano Hybrids and Composites Vol. 51
Author(s): Roshafima Rasit Ali, Justin Zhe Chan, Suriya Rajendran, Mohd Shahrul Nizam Salleh
This study investigates the green synthesis of silver nanoparticles (AgNPs) using Azadirachta indica extract and their incorporation onto orthodontic brackets, enhanced with different concentrations of Azadirachta indica extract. AgNPs exhibit strong antimicrobial activity, making them promising agents in biomedical applications. The synthesized AgNPs were characterized using UV-Vis spectroscopy, FTIR, XRD, and FESEM. The AgNPs were embedded into orthodontic brackets to assess their antimicrobial properties against Staphylococcus aureus and Escherichia coli. Characterization results confirmed the successful formation of crystalline, spherical AgNPs with sizes ranging from 30–68 nm. Antimicrobial testing revealed clear zones of inhibition around AgNP-coated brackets, demonstrating enhanced antibacterial efficacy. The study supports the potential of eco-friendly synthesized AgNPs for improved oral health in orthodontic treatments.
[1] M. A. Al-Mutairi, L. Al-Salamah, L. A. Nouri, B. S. Al-Marshedy, N. H. Al-Harbi, E. A. Al-Harabi, H. A. Al-Dosere, F. S. Tashkandi, Z. M. Al-Shabib, A. M. Altalhi. Microbial Changes in the Periodontal Environment Due to Orthodontic Appliances: A Review. 16(7). (2024) pp.5-12.
DOI: 10.7759/cureus.64396
[2] S. K. Mallineni, S. Sakhamuri, S. L. Kotha, A. R. G. M. AlAsmari, G. H AlJefri, F. N. Almotawah, S. Mallineni, & R. Sajja. Silver nanoparticles in dental applications: A Descriptive review. Bioengineering, 10(3), 327. (2023).
[3] M. A. Alzohairy. Therapeutics Role ofAzadirachta indica(Neem) and Their Active Constituents in Diseases Prevention and Treatment. Evidence-based Complementary and Alternative Medicine (2016) pp.1-11.
DOI: 10.1155/2016/7382506
[4] B. Barik, B. S. Satapathy, G. Pattnaik, D. V. Bhavrao & K. P. Shetty. Sustainable synthesis of silver nanoparticles from Azadirachta indica: antimicrobial, antioxidant and in silico analysis for periodontal treatment. Frontiers in Chemistry. (2024) pp.1-14.
[5] Jaisankar, Applications of silver nanoparticles in dentistry. International Journal of Research in Pharmaceutical Sciences, (2020) p.1126–1131.
[6] S. Ahmed. Green Synthesis of Silver Nanoparticles Using Azadirachta Indica Aqueous Leaf Extract. Vol 9 (1). 2025 pp.1-7.
[7] M. S. N. Salleh, R. R. Ali., K. Shameli, M. Y. Hamzah, R. Kasmani & M. M. Nasef. Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity. Polymers, 13(20), (2021) p.354.