Isolation and characterization of the aconitate hydratase 4 (Aco4) gene from soybean

Дата публикации: 14-08-2026 00:00:00

Aconitase catalyzes the reversible isomerization of two tricarboxylic acids, citrate and isocitrate, during the Krebs cycle. Five aconitase genes, namely, Aco1, Aco2, Aco3, Aco4, and Aco5, have been identified in soybean. Previously, Aco4 was mapped on chromosome 11. The purpose of this investigation was to isolate and sequence the candidate gene for Aco4. We mapped the Aco4 gene to a 148 kb region on chromosome 11 that contained 19 predicted genes. One of these, Glyma.11G080600, codes for aconitate hydratase. Sequencing of two isozyme variants (A-line and B-line) for Glyma.11G080600 revealed three synonymous and two non-synonymous substitutions. Perhaps, the two non-synonymous substitutions resulted in a variable isozyme pattern between the variants. Glyma.11G080600 contains a catalytic domain and a swivel domain that are known to catalyze isomerization of citrate to isocitrate and swiveling conformational change in the enzyme mechanism, respectively. Conservation of both the...

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