¹ School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Chiang Mai, 50200, Thailand
² Research Institute for Health Sciences, Chiang Mai University, Chiangmai, CHIANG MAI, 50200, Thailand
³ Psychiatry, Chiang Mai University Faculty of Medicine, Chiang Mai, Chiang Mai, 50200, Thailand

Kyaw Sein Lynn
Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Validation, Writing – Original Draft Preparation

Linda Aurpibul
Roles: Conceptualization, Data Curation, Investigation, Methodology, Project Administration, Supervision, Validation, Writing – Review & Editing

Nuntisa Chotirosniramit
Roles: Conceptualization, Investigation, Resources, Visualization, Writing – Review & Editing

Natthapol Kosashunhanan
Roles: Methodology, Resources, Supervision, Visualization, Writing – Review & Editing

Kriengkrai Srithanaviboonchai
Roles: Resources, Supervision, Visualization, Writing – Review & Editing

Awirut Oon-arom
Roles: Formal Analysis, Methodology, Supervision, Visualization, Writing – Review & Editing

PrEP was first introduced in Thailand in 2016 and has subsequently become an integral component of the national HIV prevention programme (Phanuphak et al., 2018). PrEP is available through public hospitals, private hospitals, and community-led health services for individuals at risk of HIV acquisition, including key populations. This cross-sectional study was conducted in Chiang Mai, Northern Thailand in 2025. At the time of this study, PrEP services are available at more than 20 government hospitals, more than 10 private hospitals, and several community-led clinics (Data Center, Chiang Mai Provincial Health office, 2025). Among these, PIMAN Clinic, a university-affiliated research clinic, and MPLUS Polyclinic, a Key Population-Led Health Service (KPLHS) operated by a community-based organization, were selected because they are the principal PrEP service providers for MSM and TGW in Chiang Mai. Located approximately 7 km apart, these clinics serve approximately 850 active PrEP clients and represent Thailand’s two major PrEP service delivery models: healthcare facility-based and community-led services. Participants in the two clinics represented the key population residing in urban area and had comparable demographics; some of them alternated between the two clinics.

To align with the programmatic eligibility criteria for PrEP services, the study population comprised individuals assigned male at birth and transgender women who reported sexual activity with men. The study was designed to compare two predefined exposure groups: participants who had used PrEP continuously for at least six months and participants who had never used PrEP. Individuals who had previously initiated but discontinued PrEP were excluded because they did not meet either predefined exposure category.

Participants were recruited after completing their routine clinic services. At both study sites, HIV testing or HIV status verification was routinely performed according to standard clinical protocols before recruitment into HIV prevention services. Consequently, recruitment was restricted to clients eligible for HIV prevention services, and individuals living with HIV were not approached for enrolment.

Recruitment was conducted through a purposive non-probability sampling strategy, aiming for a total sample size of 150 participants. A sample size of 150 participants was selected based on the commonly reported depression prevalence of approximately 35% among MSM (Nouri et al., 2022). With this sample, the 95% confidence interval around a 35% prevalence estimate would have a margin of error of about ±8%, which was considered adequate for descriptive purposes. The study was therefore considered adequately powered for descriptive analyses and exploratory analyses of associations between PrEP use and mental health outcomes.

Recruitment was conducted between March and May 2025. A total of 150 participants were enrolled, comprising 75 PrEP users and 75 non-users. Although 75 participants were recruited from each clinic, recruitment site was recorded only for PrEP users because this information was not captured on the questionnaire for non-users. Inclusion criteria were: (1) aged ≥20 years; (2) being an individual assigned male at birth who reported sexual activity with men or identifying as a transgender woman; and (3) either a current PrEP user with at least six months of continuous use or an individual who had never used PrEP. Trained research assistants screened clinic appointment logs, approached eligible clients in waiting areas, obtained written informed consent, and administered structured questionnaires in private consultation rooms. The following variables and measures were collected.

Sociodemographic variables included age, self-identified gender identity (male, female, transgender woman, or non-binary), education level, living arrangement, employment status, and monthly income.

Perceived community safety was assessed using the question, “Do you feel safe in your current living community?” Responses were dichotomized as “Always safe” and “Not always safe.” Current stress was assessed using a single-item self-report question: “How would you rate your current stress level?” Responses were dichotomized as “High stress” and “Low/no stress.” LGBTQ+ community involvement was assessed by asking whether participants actively participated in LGBTQ+-related community groups or activities (Yes/No).

Some participants self-identified as female or non-binary; however, they were included because they met the behavioural eligibility criteria for MSM or TGW. This approach is consistent with Thailand’s PrEP programme, which defines key populations primarily according to behavioural risk and gender identity relevant to HIV prevention services rather than identity labels alone.

Depression severity was measured using the Patient Health Questionnaire-9 (PHQ-9) (Kroenke et al., 2001). The PHQ-9 consists of nine items assessing depressive symptoms during the previous two weeks using a four-point Likert scale ranging from 0 (“Not at all”) to 3 (“Nearly every day”). Total scores range from 0 to 27, with higher scores indicating more severe depressive symptoms.

The PHQ-9 total score was used as the primary continuous outcome in all analyses. The Thai version of the PHQ-9 has demonstrated good reliability and validity for screening major depression, with a Cronbach’s alpha of 0.79 yielding 84% sensitivity and 77% specificity (Lotrakul et al., 2008).

Suicidal ideation and risk were assessed using the Columbia-Suicide Severity Rating Scale (C-SSRS) Screen Version (Posner et al., 2011). The instrument has demonstrated good convergent and divergent validity with high sensitivity and specificity. A validated Thai version is available through the Columbia Lighthouse Project (The Columbia Lighthouse Project, 2016) Responses to the six dichotomous items (wish to be dead, suicidal thoughts, method, intent, plan, and suicidal behaviour) were recorded. Participants endorsing one or more items were classified as having suicidal ideation/risk, whereas those endorsing none were classified as having no suicidal ideation/risk.

All analyses were conducted using Jamovi version 2.7 (The jamovi Project, Sydney, Australia). Data cleaning included verification of data entry, identification of out-of-range values, and correction of entry errors where applicable.

Descriptive statistics were used to summarize participants’ sociodemographic characteristics, psychosocial factors, and mental health outcomes. Categorical variables were presented as frequencies and percentages, whereas continuous variables were summarized using means and standard deviations. Comparisons between PrEP users and non-users were performed using the χ² test (or Fisher’s exact test when expected cell counts were <5) for categorical variables and the independent-samples t-test for continuous variables. The Mann–Whitney U test was used when continuous variables were not normally distributed.

To identify factors associated with depression, multivariable linear regression analysis was performed using the continuous PHQ-9 total score as the dependent variable. Independent variables entered into the model were age group, education level, employment status, monthly income, experience of discrimination, family/societal pressure, community safety, current stress level, social support, LGBTQ+ community involvement, and PrEP use. Unstandardized regression coefficients (B), 95% confidence intervals (CIs), and p-values were reported. Model assumptions were assessed by examining residual plots for linearity and homoscedasticity, while multicollinearity was evaluated using variance inflation factors (VIFs), with values <2 indicating no evidence of problematic multicollinearity.

Because suicidal ideation/risk occurred infrequently, resulting in too few outcome events for reliable multivariable modelling, logistic regression was not performed. Instead, Fisher’s exact test was used to compare suicidal ideation/risk between PrEP users and non-users.

A total of 150 participants were included in the analysis, comprising 75 PrEP users and 75 non-users. Sociodemographic and psychosocial characteristics by PrEP use status are presented in Table 1. Of the 150 participants, the majority self-identified as MSM (76.0%), followed by transgender women (16.0%), non-binary individuals (5.3%), and a small proportion biological males who identified themselves as female (2.7%).

Compared with non-users, PrEP users were more likely to be older than 40 years (13.3% vs. 2.7%, p = 0.031), employed (82.7% vs. 65.3%, p = 0.025), report always feeling safe in their community (64.0% vs. 46.7%, p = 0.048), and actively participate in LGBTQ+ community activities (88.0% vs. 69.3%, p = 0.009). High stress was reported only among non-users (9.3% vs. 0.0%, p = 0.013). No statistically significant differences were observed for education level, living arrangement, monthly income, experience of discrimination, family/societal pressure, or social support (all p > 0.05).

Mental health outcomes according to PrEP use are presented in Table 2. PrEP users had significantly lower mean depression scores than non-users (3.80 ± 3.46 vs. 5.96 ± 3.76), with a mean difference of 2.16 (95% CI: 0.99–3.33; p < 0.001).

Suicidal ideation/risk was reported by 5 (6.7%) PrEP users and 3 (4.0%) non-users. Fisher’s exact test showed no statistically significant difference between the two groups (p = 0.719).

A multivariable linear regression model was performed to identify factors associated with depression scores (Table 3). The multivariable model explained 17.1% of the variance in depression scores (R² = 0.171; adjusted R² = 0.098). After adjustment for demographic and psychosocial factors, participants who did not report experiencing discrimination had significantly lower depression scores than those who had experienced discrimination (B = −1.32, 95% CI: −2.59 to −0.06; p = 0.040). PrEP use was independently associated with lower depression scores (B = −1.77, 95% CI: −3.07 to −0.47; p = 0.008). Age group, education level, employment status, monthly income, family/societal pressure, community safety, current stress level, social support, and LGBTQ+ community involvement were not significantly associated with depression scores (all p > 0.05).

Suicidal ideation was reported by 8 of the 150 participants (5.3%). Among PrEP users, 5 participants (6.7%) reported suicidal ideation compared with 3 participants (4.0%) among non-users (Table 4). Fisher’s exact test showed no statistically significant association between PrEP use and suicidal ideation (p = 0.719).

The substantial burden of depressive symptoms observed in this study is consistent with previous research demonstrating disproportionately high rates of depression among MSM and TGW. A 2022 meta-analysis estimated that depression among MSM was approximately threefold higher than in the general male population, highlighting the persistent mental-health inequality faced by this group (Nouri et al., 2022). Longitudinal research among MSM without HIV in China has also shown that anxiety and depression are highly prevalent and remain elevated over time, underscoring the chronic nature of psychological distress in MSM communities (Wu et al., 2022). Among transgender women, high levels of depression have been reported in several Asian settings. A study of trans women in Bangkok found that more than half met criteria for depression, and low perceived social support was strongly associated with depressive symptoms, which is similar to the pattern of stress and support observed in our data (Tantirattanakulchai & Hounnaklang, 2021).

The lower depression scores among PrEP users in our study are in line with emerging literature on the mental-health dimensions of PrEP. A 2023 study examining PrEP, anxiety, and depression among MSM found that mental-health concerns such as depression and anxiety were closely related to PrEP use patterns and impacted broader quality of life and sexual satisfaction, suggesting that PrEP use occurs within a broader psychosocial context rather than being a purely biomedical intervention (Reiriz et al., 2023). More recently, a 2024 study of Chinese MSM initiating PrEP showed distinct trajectories of anxiety and depression during PrEP use and concluded that mental-health support should be integrated into PrEP programs to sustain adherence and well-being (Chen et al., 2024).

In the present study, PrEP use remained independently associated with lower depression scores after adjustment for demographic and psychosocial factors. However, because of the cross-sectional design, these findings should be interpreted as associations rather than evidence of causality. Individuals with better baseline psychosocial functioning may have been more likely to access, initiate, and remain engaged in PrEP services, and reverse causality cannot be excluded. Longitudinal studies are needed to clarify the temporal relationship between PrEP engagement and mental health outcomes.

Experience of discrimination was independently associated with higher depression scores in the adjusted analysis. This finding is consistent with minority stress theory, which proposes that stigma and discrimination contribute to adverse mental health outcomes among sexual and gender minority populations (Pellicane & Ciesla, 2022). Persistent experiences of discrimination may increase psychological distress through chronic exposure to social exclusion, prejudice, and reduced access to supportive environments. This finding further emphasizes the importance of addressing discrimination as part of broader strategies to improve mental health among sexual and gender minority populations.

Beyond discrete experiences of discrimination, broader dimensions of stigma are also likely relevant to depression in this population; a composite stigma score collected alongside this study showed a modest but statistically significant correlation with depression score (r = 0.246, p = 0.002), consistent with minority stress theory, and is explored in greater depth in a companion analysis.

Suicidal ideation/risk was relatively uncommon in this study and did not differ significantly between PrEP users and non-users. Because only eight participants reported suicidal ideation/risk, the study had limited statistical power to detect between-group differences. Nevertheless, suicidal ideation among MSM and TGW remains an important public health concern, and routine screening may still be warranted within HIV prevention services.

A major strength of this study was the use of validated Thai versions of the PHQ-9 and C-SSRS to assess mental health outcomes among MSM and TGW. In addition, the study compared PrEP users and non-users recruited from two major HIV prevention service providers in Chiang Mai and adjusted for a range of demographic and psychosocial covariates when examining factors associated with depression.

This study has several limitations. First, its cross-sectional design precludes causal inference, and reverse causality cannot be excluded. Individuals with better baseline psychosocial functioning may have been more likely to access and remain engaged in PrEP services.

Second, the study compared long-term PrEP users (≥6 months) with individuals who had never used PrEP. Participants who discontinued PrEP before six months were excluded, which may have introduced survivor bias.

Third, MSM and TGW were analysed together because the number of TGW participants was insufficient to support reliable subgroup analyses. These populations experience distinct forms of minority stress, and future studies with larger samples should examine them separately.

Fourth, recruitment site could not be adjusted for because recruitment site information was not captured. Residual confounding related to differences between service settings therefore cannot be excluded.

Fifth, a comprehensive 28-item stigma scale (capturing enacted, perceived, and internalized stigma) was also collected in the parent study but was not entered as a covariate in the present multivariable model, as it falls outside this study’s pre-specified focus on demographic and psychosocial correlates of depression. An exploratory bivariate analysis showed a statistically significant, modest correlation between the composite stigma score and depression score (Pearson r = 0.246, p = 0.002; shared variance ≈ 6%), consistent with stigma being related to — but not the dominant driver of — depressive symptoms in this sample, and unlikely to have materially confounded the PrEP–depression association reported here. The full stigma scale, including its subscales, is examined in depth in a separate manuscript currently in preparation.

Finally, suicidal ideation/risk was uncommon (n = 8), limiting statistical power to detect associations.

The data that support the findings of this study can be accessed from Zenodo repository. Dataset_depression and suicidal ideation in Thai MSM [Data set]. Zenodo. https://doi.org/10.5281/zenodo.20061760 (Lynn, K. S., 2026).

The author thanks the staff and participants at PIMAN Clinic and MPLUS Polyclinic for their support and cooperation.

An earlier version of this work was posted as a preprint on https://doi.org/10.21203/rs.3.rs-7176096/v1;

However, the current manuscript has undergone substantial revision including updated analyses and interpretation of findings.

•  Chaovanalikit T, Wirairat K, Sriswadpong P: quality of life, self-esteem, and depression among thai transgender women before and after male-to-female gender confirmation surgery: A prospective cohort observational study. Sex Med. 2022; 10(4): 100533. PubMed Abstract | Publisher Full Text | Free Full Text
•  Chautrakarn S, Rayanakorn A, Intawong K, et al.: PrEP stigma among current and non-current PrEP users in Thailand: A comparison between hospital and key population-led health service settings. Front. Public Health. 2022; 10: 1019553. Publisher Full Text
•  Chen S, Zhu YY, Chu ZX, et al.: Trajectories of anxiety and depression among Chinese men who have sex with men on pre-exposure prophylaxis: a group-based trajectory model approach. BMC Public Health. 2024; 24(1): 365. Publisher Full Text
•  The Columbia Lighthouse Project: 2016. Retrieved 13 Sep 2025. Reference Source
•  Data Center, Chiang Mai Provincial Health office: 2025. Retrieved 13 Sep 2025. Reference Source
•  Davies O, Ustianowski A, Fox J: Pre-exposure prophylaxis for HIV prevention: why, what, who and how. Infect. Dis. Ther. 2016; 5: 407–416. PubMed Abstract | Publisher Full Text | Free Full Text
•  Hennessy F, Turner L, Higgins V, et al.: 1539. Presence of mental health concerns is associated with poor adherence in people taking pre-exposure prophylaxis in real-world clinical practice in the United States. Open Forum Infect. Dis. 2023; 10(Suppl 2). ofad500.1374 [pii]. Publisher Full Text
•  Kroenke K, Spitzer RL, Williams JB: The PHQ-9: validity of a brief depression severity measure. J. Gen. Intern. Med. 2001; 16(9): 606–613. PubMed Abstract | Publisher Full Text | Free Full Text
•  Lotrakul M, Sumrithe S, Saipanish R: Reliability and validity of the Thai version of the PHQ-9. BMC Psychiatry. 2008; 8: 46. PubMed Abstract | Publisher Full Text | Free Full Text
•  Lynn KS: Dataset_depression and suicidal ideation in Thai MSM. [Data set]. Zenodo. 2026. Publisher Full Text
•  Manges ME, Bryan C, Bryan A, et al.: Suicidal thoughts and behaviors among gender and sexual minorities: Adults ages 18-24 show highest rates of past month suicidal thoughts. Suicide Life Threat. Behav. 2024; 54(6): 904–913. Publisher Full Text
•  Miller SJ, Harrison SE, Sanasi-Bhola K: A scoping review investigating relationships between depression, anxiety, and the PrEP care continuum in the United States. Int. J. Environ. Res. Public Health. 2021; 18(21). PubMed Abstract | Publisher Full Text | Free Full Text
•  Naswa S, Marfatia YS: Pre-exposure prophylaxis of HIV. Indian journal of sexually transmitted diseases. 2011; 32: 1–8. Publisher Full Text
•  Nouri E, Moradi Y, Moradi G: What is the global prevalence of depression among men who have sex with men? A systematic review and meta-analysis. Ann. General Psychiatry. 2022; 21(1): 38. PubMed Abstract | Publisher Full Text | Free Full Text
•  Operario D, Sun S, Bermudez AN, et al.: Integrating HIV and mental health interventions to address a global syndemic among men who have sex with men. Lancet HIV. 2022; 9(8): e574–e584. PubMed Abstract | Publisher Full Text | Free Full Text
•  Pellicane MJ, Ciesla JA: Associations between minority stress, depression, and suicidal ideation and attempts in transgender and gender diverse (TGD) individuals: Systematic review and meta-analysis. Clin. Psychol. Rev. 2022; 91: 102113. PubMed Abstract | Publisher Full Text
•  Phanuphak N, Sungsing T, Jantarapakde J, et al.: Princess PrEP program: the first key population-led model to deliver pre-exposure prophylaxis to key populations by key populations in Thailand. Sex. Health. 2018; 15(6): 542–555. PubMed Abstract | Publisher Full Text
•  Posner K, Brown GK, Stanley B, et al.: The columbia-suicide severity rating scale: initial validity and internal consistency findings from three multisite studies with adolescents and adults. Am. J. Psychiatry. 2011; 168(12): 1266–1277. PubMed Abstract | Publisher Full Text | Free Full Text
•  Protiere C, Sagaon-Teyssier L, Donadille C, et al.: Perception of PrEP-related stigma in PrEP users: Results from the ANRS-PREVENIR cohort. HIV Med. 2023; 24(8): 938–945. Publisher Full Text
•  Reiriz M, Rodríguez-Expósito B, Jiménez-García AJ, et al.: Pre-exposure prophylaxis, anxiety, depression and sexual satisfactionamong men who have sex with men. Psicothema. 2023; 2(2): 159–169. PubMed Abstract | Publisher Full Text
•  Wood SM: Mental health, social influences, and HIV Pre-exposure prophylaxis (PrEP) utilization among men and transgender individuals screening for HIV prevention trials. PubMed. 2021. Reference Source
•  Sun CJ; A. KToevs K, et al.: “Little tablets of gold”: An examination of the psychological and social dimensions of PrEP among LGBTQ communities. AIDS Educ. Prev. 2019; 31: 51–62. Publisher Full Text Reference Source
•  Tantirattanakulchai P, Hounnaklang N: Perceived social support and its relationship with depression among Bangkok’s trans women. Journal of Health Research. 2021; 36(2): 365–375. Publisher Full Text
•  Tomori C, McFall AM, Srikrishnan AK, et al.: Diverse rates of depression among men who have sex with men (MSM) across India: Insights from a Multi-site Mixed Method Study. AIDS Behav. 2016; 20(2): 304–316. PubMed Abstract | Publisher Full Text | Free Full Text
•  Turner L, Roepke A, Wardell E, et al.: Do You PrEP? A review of primary care provider knowledge of PrEP and attitudes on prescribing PrEP. J. Assoc. Nurses AIDS Care. 2018; 29(1): 83–92. PubMed Abstract | Publisher Full Text | Free Full Text
•  Vadhanavikkit P, Srifuengfung M, Wiwattarangkul T, et al.: Prevalence of depression and generalized anxiety among LGBTQ+ medical students in Thailand. J. Homosex. 2025; 72(9): 1751–1771. PubMed Abstract | Publisher Full Text
•  Vannakit R, Janyam S, Linjongrat D, et al.: Give the community the tools and they will help finish the job: key population-led health services for ending AIDS in Thailand. J. Int. AIDS Soc. 2020; 23(6): e25535. PubMed Abstract | Publisher Full Text | Free Full Text
•  Wilton L, Chiasson MA, Nandi V, et al.: Characteristics and correlates of lifetime suicidal thoughts and attempts among young black men who have sex with men (msm) and transgender women. J. Black Psychol. 2018; 44: 273–290. Publisher Full Text
•  Wu D, Zhong X, Deng R, et al.: Study on anxiety and depression of men who have sex with men: An application of group-based trajectory model. Front. Psychol. 2022; 13: 857203. PubMed Abstract | Publisher Full Text | Free Full Text
•  Yigit I, Budhwani H, Rainer CB, et al.: Associations between PrEP stigma, PrEP confidence, and PrEP adherence: Conditional indirect effects of anticipated HIV stigma. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2022; 10: 1097.

Published: 11 Aug 2026, 15:772

Published: 10 Jul 2026, 15:772

Published: 21 May 2026, 15:772

Current Reviewer Status: ?

VERSION 2

PUBLISHED 10 Jul 2026

Revised

Reviewer Report 16 Jul 2026

Ashok Jammigumpula, Manipal Academy of Higher Education, Manipal, Karnataka, India 

Not Approved

VIEWS 0

VERSION 1

PUBLISHED 21 May 2026

Reviewer Report 26 Jun 2026

Ashok Jammigumpula, Manipal Academy of Higher Education, Manipal, Karnataka, India 

Not Approved

VIEWS 0

Reviewer Report 01 Jun 2026

Francesca Vedovo, University of Trieste, Trieste, Italy 

Approved with Reservations

VIEWS 0